Fig. 1. Oxidative stress measurement in endothelial cells during zinc deficiency and supplementation and after exposure to linoleic acid (FA) or TNF. Cells were cultured for 8 days in media supplemented with only 1% FBS and subsequently enriched either with zinc (10 µM) for 48 hours and/or treated with FA (90 µM) for 6 hours or TNF (500 U/mL) for 1.5 hours. Values are mean±SEM (n=6). *Significantly different from respective cultures without added zinc.