Fig. 2. Effect of linoleic acid (FA) and added zinc on activation of NF-B. Cells were cultured for 8 days in media supplemented with 1% FBS and appropriate groups enriched with zinc (10 µM) for 48 hours and treatment with FA (90 µM) for 6 hours. Lane 1 = positive control (HeLa cell extract); lane 2 = competitor (unlabeled oligonucleotide); lane 3 = cells cultured in M199 enriched with 1% FBS (control); lane 4 = control + zinc (10 µM); lane 5=control + FA (90 µM); lane 6=control + zinc + FA.