Fig. 3. Effect of wortmannin treatment on the stimulation of glycogen production in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with insulin (100 nM), MHCP (0.1 mg/mL) or insulin + MHCP in combination with various concentrations of wortmannin for 30 minutes and washed. The incorporation of D-[¹⁴C]glucose into the glycogen pools (without wortmannin present) was allowed for 60 minutes. The radioactivity incorporated into the glycogen was measured in the glycogen precipitate. The data represent the mean ± standard deviation of triplicate samples from three experiments.