Fig. 4. Effect of wortmannin on the stimulation of glycogen production in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with various concentrations of wortmannin in the presence of insulin (100 nM), MHCP (0.1 mg/mL) or insulin + MHCP for 30 minutes. The incorporation of D-[¹⁴C]glucose into the glycogen pools in the presence of wortmannin was allowed for 60 minutes. The radioactivity incorporated into the glycogen was measured in the glycogen precipitate. The data represent the mean ± standard deviation of triplicate samples from two experiments.