Fig. 4. Analysis of seeds from plants transformed with the fungal phytase (P1, P2, P3, P4) and an untransformed control (WT). (a) The phytase activity was measured at pH 6.5 and 37°C before and after acid or heat treatment. (b) The phytate content from seeds of a transgenic plant (P4) and an untransformed control was determined by HPLC analysis before (1) and after (2) simulated small intestine conditions. [42 (Reproduced with permission.)]