Fig. 2. Leptin levels before and after the carbohydrate and the fat meal in lean and obese women and during the fasting experiment. Statistical analyses were carried out separately in lean and obese subjects. Two-way ANOVA with "time" (repeated factor 10 levels: 0 to 9 hours) and "meal" (repeated measures: carbohydrate/fat/fast) and their interaction was used for statistical analyses. Statistically significant (p < 0.001) interactions were found between the two factors in both lean and obese subjects.