Fig. 6. Effect of leukotoxin (LTX) and leukotoxin diol (LTD) on the activation of NF-B (A) and AP-1 (B) in porcine pulmonary artery endothelial cells. Confluent monolayers were treated with LTX (60 and 90 µM) and LTD (60 and 90 µM) for six hours. Fig. 5A: lane 1, control; lane 2, LTX (60 µM); lane 3, LTX (90 µM); lane 4, LTD (60 µM); lane 5, LTD (90 µM) and lane 6, LTX (90 µM) supershift (p65). Fig. 5B: lane 1, control; lane 2, LTX (60 µM); lane 3, LTX (90 µM); lane 4, LTD (60 µM); lane 5, LTD (90 µM) and lane 6, LTX (90 µM) supershift (c-jun). Values are mean ± SEM of three separate experiments. *Significantly different from control values.