Fig. 7. EPA prevents LPS-induced I
B-
phosphorylation and degradation. THP-1 cells pre-incubated with or without 60 µM of EPA for 24 hours were stimulated with 0.2 µg/mL of LPS for various time periods. Cytosolic extracts were prepared and analyzed by Western Blot using antibodies specifically recognize I
B-
(A) and phosphorylated I
B-
(B). Data are representative of three experiments.