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Fig. 7. EPA prevents LPS-induced I{kappa}B-{alpha} phosphorylation and degradation. THP-1 cells pre-incubated with or without 60 µM of EPA for 24 hours were stimulated with 0.2 µg/mL of LPS for various time periods. Cytosolic extracts were prepared and analyzed by Western Blot using antibodies specifically recognize I{kappa}B-{alpha} (A) and phosphorylated I{kappa}B-{alpha} (B). Data are representative of three experiments.