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Original Research |
Discipline of Nutrition and Dietetics, University of Newcastle (L.G.W., M.L.G.), John Hunter Children’s Hospital, Newcastle
Department of Paediatrics (D.A.F., D.M.C.), John Hunter Children’s Hospital, Newcastle
Airway Research Centre, Department of Respiratory Medicine (P.G.G.), John Hunter Children’s Hospital, Newcastle
Department of Dietetics (C.E.C.), John Hunter Children’s Hospital, Newcastle
Department of Respiratory Medicine, New Children’s Hospital, Sydney (D.A.F.), New South Wales, AUSTRALIA
Address reprint requests to: Dr Manohar Garg, Head of Discipline of Nutrition and Dietetics, Faculty of Medicine & Health Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia. E-Mail: ndmg{at}medicine.newcastle.edu.au.
Objective: To examine oxidative stress in CF by measuring 8-iso-PGF2
and antioxidant defenses, in relation to dietary intake, immune function and clinical status.
Methods: We measured total plasma concentrations of 8-iso-PGF2 and dietary antioxidants (vitamin E, vitamin C, ß-carotene), erythrocyte antioxidant enzyme activities (glutathione peroxidase and superoxide dismutase), lung function and dietary intake in 21 CF subjects and 21 healthy age- and gender-matched controls.
Results: Total plasma 8-iso-PGF2 concentration (median [quartile 1–quartile 3]) was significantly higher in CF subjects compared to controls (214 pg/mL (155–331) vs. 135 pg/mL (101–168), p=0.001). Neutrophil, monocyte and total white cell counts were elevated in the CF group and these correlated with 8-iso-PGF2 concentration. Despite similar dietary intake, lower plasma antioxidant concentrations were observed in the CF group (vitamin E, p < 0.001, vitamin C, p=0.004, ß-carotene, p=0.001). 8-iso-PGF2 correlated negatively with plasma vitamin E, C and ß-carotene concentrations.
Conclusion: Oxidative stress is increased in CF patients, despite normal dietary antioxidant intake. The immune response appears to be a key factor causing oxidative stress. Antioxidant intervention aimed at reducing oxidative stress in CF needs to be assessed.
Key words: cystic fibrosis, oxidative stress, antioxidants, isoprostanes
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