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Journal of the American College of Nutrition, Vol. 23, No. 5, 552S-555S (2004)
Published by the American College of Nutrition

The Determination of Brain Magnesium and Zinc Levels by a Dual-Probe Microdialysis and Graphite Furnace Atomic Absorption Spectrometry

Dar-Yu Yang, MD, MSC, Jen-Bin Lee, MS, Ming-Cheng Lin, MS, Yeou-Lih Huang, PhD, Hong-Wen Liu, MD, Yea-Jiuan Liang, MS and Fu-Chou Cheng, PhD

Department of Emergency (D.Y.Y.), Kaoshiung Medical University, Kaoshiung, TAIWAN
Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN
Taichung Veterans General Hospital, Department of Applied Chemistry, Providence University (J.B.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN
Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN
Graduate Institute of Medicine, (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN
School of Technology for Medical Sciences (Y.L.H.), Kaoshiung Medical University, Kaoshiung, TAIWAN

Address reprint requests to: Dr. Fu-Chou Cheng, Department of Medical Research, Taichung Veterans General Hospital, Taichung, 40705, TAIWAN. E-mail: fucheng{at}vghtc.gov.tw

Objective: The aim of this study was to develop a microdialysis-graphite furnace atomic absorption spectroscopy (MD-GFAAS) for monitoring dynamic changes of extracellular magnesium (Mg) and zinc (Zn) in the cortex of gerbils subjected to focal cerebral ischemia, that had been produced in anesthetized gerbils by occlusion of the right middle cerebral artery.

Methods: Two microdialysis probes were inserted into both sides of the cortex to simultaneously collect dialysates during cerebral ischemia. Dynamic changes in these analytes, on ipsilateral and contralateral sides of the brain, were assayed by MD-GFAAS. Optimal conditions and analytical precision of GFAAS were studied in the present assay.

Results: The present study demonstrated significant decreases in Mg (65% of baseline) and zinc (74% of baseline) maintained their levels within 3 h on the ipsilateral side of cortex during cerebral ischemia. Slight changes of Mg and Zn on the contralateral sides were also observed.

Conclusion: The derangement of extracellular Mg and Zn could be important in the progression of cell injury and may be associated with cerebral ischemia insult.

Key words: cerebral ischemia, magnesium, zinc, dual-probe, gerbil, microdialysis, GFAAS






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Copyright © 2004 by the American College of Nutrition.