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Journal of the American College of Nutrition, Vol 7, Issue 6 491-497, Copyright © 1988 by American College of Nutrition

JOURNAL ARTICLE

HPLC analysis of the phenylisothiocyanate (PITC) derivatives of taurine from physiologic samples

S. E. Lippincott, A. L. Friedman, F. L. Siegel, R. M. Pityer and R. W. Chesney
Department of Pediatrics, University of Wisconsin Hospital and Clinics, Madison 53792.

Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.




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Copyright © 1988 by the American College of Nutrition.