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Ingestion of a Dietary Supplement Containing Dehydroepiandrosterone (DHEA) and Androstenedione Has Minimal Effect on Immune Function in Middle-Aged Men

Department of Health and Human Performance, Iowa State University, Ames, Iowa

View larger version (16K): [in a new window]   Fig. 1. PHA-induced proliferation in PBMC from middle-aged subjects consuming either placebo or supplement and young subjects that did not consume placebo or supplement. Results are expressed as mean ± SEM. Asterisk indicates significant differences (p < 0.05) between placebo and supplement.

View larger version (18K): [in a new window]   Fig. 2. PHA-induced production of the Th1 cytokines, IL-2 and IFN- in supernatant from PBMC cultured with PHA at 5 µg/mL. PBMC were obtained from middle-aged subjects consuming either placebo or supplement, or from young subjects consuming no supplement at the time points indicated. Results are expressed as mean ± SEM.

View larger version (18K): [in a new window]   Fig. 3. PHA-induced production of the Th2 cytokines, IL-4 and IL-10 in supernatant from PBMC cultured with PHA at 5 µg/mL. PBMC were obtained from middle-aged subjects consuming either placebo or supplement, or from young subjects that did not consume placebo or supplement at the time points indicated. Results are expressed as mean ± SEM.

View larger version (15K): [in a new window]   Fig. 4. LPS-induced production of IL-1ß in supernatant from PBMC culture with LPS at 100 ng/mL. PBMC were obtained from middle-aged subjects consuming either placebo or supplement at the time points indicated. Results are expressed as mean ± SEM.

View larger version (48K): [in a new window]   Fig. 5. PBMC obtained from middle-aged men and incubated with PHA (5 µg/mL) plus media, the dietary supplement (containing androstenedione and DHEA at 10-7 M), androstenedione (10-7 M) or DHEA (10-7 M). Results are expressed as mean ± SEM.

View larger version (42K): [in a new window]   Fig. 6. IL-2 and IFN- in supernatant from PBMC obtained from middle-aged men and incubated with PHA (5 µg/mL) plus media, the dietary supplement (containing androstenedione and DHEA at 10-7 M), androstenedione (10-7 M) or DHEA (10-7 M). Results are expressed as mean ± SEM.

View larger version (33K): [in a new window]   Fig. 7. IL-10 and IL-4 in supernatant from PBMC obtained from middle-aged men and incubated with PHA (5 µg/mL) plus media, the dietary supplement (containing androstenedione and DHEA at 10-7 M), androstenedione (10-7 M) or DHEA (10-7 M). Results are expressed as mean ± SEM. Asterisk indicates significant difference (p < 0.05) between cultures containing media alone and cultures containing dietary supplement. A trend (p < 0.1) towards a difference between cultures containing media alone and cultures containing DHEA or androstenedione is indicated by +.