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On-Line Microdialysis-Graphite Furnace Atomic Absorption Spectrometry in the Determination of Brain Magnesium Levels in Gerbils Subjected to Cerebral Ischemia/Reperfusion

Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology (M.C.L.), TAIWAN
Department of Emergency (D.Y.Y.), Taichung Veterans General Hospital, TAIWAN
Medical Research (C.P.L., L.L.Y., F.C.C.), Taichung Veterans General Hospital, TAIWAN
Department of Applied Chemistry, Providence University (C.P.L.), Taichung, TAIWAN
Taiwan Graduate Institute of Medicine, (M.C.L.), TAIWAN
School of Technology for Medical Sciences (Y.L.H.), Kaoshiung Medical University, TAIWAN
Department of Internal Medicine, Kaoshiung Medical University Hospital (H.W.L.), Kaoshiung TAIWAN

View larger version (107K): [in a new window]   Fig. 1. A schematic diagram of the on-line microdialysis coupled graphite furnace atomic absorption spectrometry system for the determination of extracellular Mg levels in a gerbil subjected to cerebral ischemia. A = microinjection pump, B = microdialysis probe, C = stereotaxic apparatus, D = diluent, E = atomic absorption spectrophotometer, F = graphite tube, G = integrated computer system.

View larger version (15K): [in a new window]   Fig. 2. Time profiles of the changes in Mg concentrations (expressed as % of basal values) in the dialysates from gerbil cortices during 60 min CCA + MCA occlusion and 1 h reperfusion. Data are presented as mean ± SEM (n = 6).