Investigation of Intracellular Magnesium Mobilization Pathways I Pc12 Cells B Simultaneous Mg-Ca Fluorescent Imaging
Takeshi Kubota, MS,
Yutaka Shindo, BT,
Kentaro Tokuno, BT,
Hirokazu Komatsu, MT,
Hiroto Ogawa, PhD,
Yoshiichiro Kitamura, PhD,
Koji Suzuki, PhD and
Kotaro Oka, PhD
School of Fundamental Science and Technology (T.K., Y.K., K.O.)
Department of System Design Engineering (Y.S., K.T., K.S.)
Department of Applied Chemistry (H.K.) Keio University
Department of Biology, Saitama Medical School (H.O.)
Department of Biosciences and Informatics (Y.K., K.O.), Keio University, Yokohama, JAPAN
View larger version (23K):
[in a new window]
|
Fig. 1. Mg is not released from ER through ryanodine receptors. Caged Ca was uncaged in both KMG-104 and fura-2 loaded PC12 cells. KMG-104 was excited at 490 nm, and fura-2 was at 380 nm wavelength. Laser flashes induced [Ca] i increase (triangles), but [Mg] i did not change (circles). Decrease of fura-2 fluorescence excited at 380 nm wavelength implies [Ca] i increase. Arrowheads indicate photolysis flashes.
|
|
View larger version (22K):
[in a new window]
|
Fig. 2. [Mg] i increased when mitochondrial activity was inhibited. Double stained PC12 cells were treated by mitochondrial inhibitor, FCCP. Bath application of 3 µM FCCP induced both [Mg] i (triangles) and [Ca] i (circles) increase. Excitation wavelengths are 490 nm for KMG-104 and 380 nm for fura-2. Decrease of fura-2 fluorescence implies [Ca] i increase. Bar indicates FCCP application.
|
|
Copyright © 2004 by the American College of Nutrition.
