Chronic Zinc Deficiency in Mice Disrupted T Cell Lymphopoiesis and Erythropoiesis While B Cell Lymphopoiesis and Myelopoiesis Were Maintained

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Chronic Zinc Deficiency in Mice Disrupted T Cell Lymphopoiesis and Erythropoiesis While B Cell Lymphopoiesis and Myelopoiesis Were Maintained

Louis E. King, PhD, Joseph W. Frentzel, James J. Mann and Pamela J. Fraker, PhD

Department of Biochemistry & Molecular Biology (L. E. K., J. W. F., P. J. F.) and *Cell and Molecular Biology Program (J. J. M.), Michigan State University, East Lansing, Michigan

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Fig. 1. Growth of mice fed synthetic zinc adequate and zinc deficient diets. In two experiments mice weighing 16.0 ± 0.7g were divided into two groups receiving either a zinc adequate (30 mg Zn/kg) or zinc deficient ( $≤$ 0.6 mg Zn/kg) diet in a 50 d dietary regime in which mice receiving zinc deficient diet developed ChrZD. Values are expressed as mean ± SD plotted at each growth point for each experiment. For each experiment ZA n = 9 ChrZD n = 8. *significantly different from ZA mice, p < 0.05.

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Fig. 2. Effect of ChrZD on the phenotypic composition of the thymus in mice at d 50. The phenotypic composition of individual mice are presented by T cell subset. Pro-T (CD90.2⁺CD4^–CD8^–), Pre-T (CD90.2⁺CD4⁺CD8⁺), CD4 (CD90.2⁺CD4⁺CD8^–), CD8 (CD90.2⁺CD4^–CD8⁺). Each bar is the group mean ± SD. ZA n = 9, ChrZD n = 8. *significantly different from ZA mice, p < 0.05."

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Fig. 3. Assessment of apoptotic cell death in thymic cell populations at d 50 in ZA and ChrZD mice by flow cytometry. Apoptotic cell death for individual mice are presented by T cell subset: Pro-T (CD90.2⁺CD4^–CD8^–), Pre-T (CD90.2⁺CD4⁺CD8⁺), CD4 (CD90.2⁺CD4⁺CD8^–), CD8 (CD90.2⁺CD4^–CD8⁺). Each bar is the group mean ± SD. ZA n = 9, ChrZD n = 8. *significantly different from ZA mice, p < 0.05."

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Fig. 4. Effect of chronic zinc deficiency on bone marrow hematopoietic composition in mice at d 50. Bone marrow of ZA and ChrZD mice were individually phenotyped to identify five marrow populations: erythroblasts (CD31^–Ly-6C^–), lymphocytes (CD31⁺Ly-6C^–), granulocytes (CD31^–Ly-6C⁺), monocytes (CD31^–Ly-6C⁺⁺), and committed progenitors (CD31⁺Ly-6C⁺). Graphics represent the percentage composition for each population in the marrow with mean ± SD values given. ZA n = 7, ChrZD n = 8. *significantly different from ZA mice, p < 0.05."