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Fig. 6. Photomicrographs of murine melanoma (B16) cells were taken 4 days after treatment. Control culture contained cells with varied morphology (a). The melanoma cells treated with vitamin E succinate (4 µg/mL) were elongated, had long cytoplasmic processes, and were arranged alongside each other (b). Melanoma cells treated with RO20-1724, an inhibitor of cyclic nucleotide phosphodiesterase (100 µg/mL) were large and elongated, had some long processes, and were arranged alongside each other (c). A combination of RO20-1724 and vitamin E succinate (d) increased the level morphologic differentiation more than that produced by individual agents [54] x450.
