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Fig. 4. Black and white representation of color coded Doppler echocardiograms demonstrating valvular regurgitation. A wide tricuspid regurgitant jet recorded from a parasternal image of the right ventricle and pulmonary artery of a SHHF-Cu- animal (a). Mitral regurgitant jet of narrow origin and wide distribution (dotted lines) recorded in the left atrium of a SHHF-Cu- animal from a parasternal long-axis image of the left ventricle (b). The aorta demonstrated a normal flow pattern. TR=tricuspid regurgitant jet; TV=tricuspid valve; MR=mitral regurgitant jet; RV=right ventricle; RA=right atrium; LV=left ventricle; LA=left atrium; AO=aorta; PA=pulmonary artery.