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Fig. 3. Effect of linoleic acid (FA) and added zinc on activation of AP-1. Cells were cultured for 8 days in media supplemented with 1% FBS and appropriate groups enriched with zinc (10 µM) for 48 hours and treatment with FA (90 µM) for 6 hours. Lane 1=positive control (HeLa cell extract); lane 2=competitor (unlabeled oligonucleotide); lane 3=cells cultured in M199 enriched with 1% FBS (control); lane 4=control + zinc (10 µM); lane 5=control + FA (90 µM); lane 6=control + zinc + FA.
