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Fig. 7. Effect of MHCP or insulin on glycogen synthase kinase3ß activity. 3T3-L1 adipocytes were treated with insulin (100 nM), MHCP (0.1 mg/mL) or insulin + MHCP for 30 minutes, washed and the cell supernate obtained. GSK-3ß assays were performed to measure the incorporation of 32P into the phosphoglycogen synthase peptide 2 (Upstate Biotechnologies). The level of incorporation of the control cells was set at 100% and the other values were set in comparison. The data represent the mean ± standard deviation of triplicate samples from three experiments.
