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Fig. 8. Increased oxidative stress results in enhanced apoptosis in the brain tumors of mice fed an antioxidant-devoid diet. The distribution of TUNEL-positive (black label, arrow) apoptotic cells in brain tumor for control (A) and in mice on the antioxidant-devoid diet (B) is shown. Oxidized guanine residues (8-oxo-Gua), biomarkers of ROS generation, were detected in brain tumors, using specific monoclonal antibodies (C and D) or an avidin-FITC conjugate (E and F). By both methods, cells in tumors of antioxidant-depleted mice (D and F) exhibit higher levels of 8-oxo-Gua residues than do cells in the tumors of control brains (C and E).
