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Fig. 2. Effect of sulfaphenazole on cellular oxidative stress as measured by DCFH oxidation (A) and cellular glutathione status (B) in endothelial cells exposed to linoleic acid (LA). For the measurement of DCF fluorescence, cells were exposed to 90 µM LA in the presence or absence of 10 µM sulfaphenazole (SP) for three hours. Fluorescence intensity was measured using a fluorescent plate reader at an excitation wavelength of 490 nm and an emission wavelength of 525 ± 5 nm. For the measurement of intracellular glutathione, cells were treated with 90 µM LA and/or SP for six hours. The values are presented as mean ± SEM of three sets of experiments. *Significantly different from control values. #Significantly different from LA group.
