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Fig. 3. Effect of MnTMPyP on cellular oxidative stress (DCFH oxidation) (Fig. 3A) and superoxide generation (Figure 3B) in endothelial cells exposed to linoleic acid (LA). Cells were exposed to 90 µM LA in the presence or absence of 10 µM MnTMPyP for three hours. Cells were loaded with either DCFH-DA or DHE (10 µM) for the last 30 minutes of the treatment period. After staining, cells were washed with HEPES buffer (pH 7.4), and the fluorescence intensity was measured using a fluorescent plate reader at an excitation wavelength of 490 nm and an emission wavelength of 525 ± 5 nm for DCF and at an excitation wavelength of 520 ± 5 nm and an emission wavelength of 620 ± 5 nm for DHE. The values are presented as means ± SEM of three sets of experiments. *Significantly different from control values. #Significantly different from LA group.
