Click on image to view larger version.
Fig. 3. Effect of EPA on stability of TNF-
mRNA. THP-1 cells were stimulated with 0.2 µg/mL of LPS after pre-incubation with or without 60 µM of EPA for 24 hours. Actinomycin D was added to the medium after cells were stimulated with LPS for 90 minutes. Cells were collected at various time points after addition of actinomycin D, and mRNA levels of TNF- and ß-actin were determined. Relative amount of mRNA (TNF-/ß-actin) at time 0 of actinomycin D addition was used as 100%. Mean ± S.E., n = 3. There was no significant difference between the means of two groups.
