Click on image to view larger version.
Fig. 4. EPA prevents LPS-induced NF-
B activation. (A) THP-1 cells pre-incubated with or without 60 µM of EPA for 24 hours were stimulated with 0.2 µg/mL of LPS for 45 minutes. Nuclear extracts were prepared and analyzed by EMSA. Data are representative of three experiments. (B) The competition assay was carried out by incubating nuclear extracts prepared from LPS-stimulated THP-1 cells with labeled probes (1), labeled probes plus excess unlabeled probes (2), or labeled probes plus excess mutated oligonucleotide (3). The mutated oligonucleotide has a "G"
"C" substitution in the NF-B/Rel DNA binding motif as described in "Material and Methods". (C) Supershift assay of NF-B. Antibodies of p65, p50 or control IgG was incubated with nuclear extracts of LPS-stimulated THP-1 cells and EMSA was performed.
