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Fig. 2. A. Western blot analysis of wild-type human {alpha}-synuclein holoprotein in undifferentiated (U) and differentiated (D) NBP2, NBP2-PN1 and NBP2-PN54 cells. 20 µg total cellular protein, anti-{alpha}-synuclein antibody, and goat anti-rabbit secondary antibody were used for the analysis. Human {alpha}-synuclein was detected as a 19 kDa protein. M-marker. B. Levels of cyclophilin A (Cyp-A) were determined and used as a loading control. Each experiment was repeated twice and similar results were obtained.





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