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Fig. 5. Photomicrographs of differentiated NB cells. Cells (20,000 cells/well) were plated in a 24 well tissue culture plate and treated 24 hours later as described below. NBP2 (A), NBP2-PN1 (B), and NBP2-PN54-C20 (C) cells differentiated for 3 days with 200 µg/ml RO20-1724 and 2 µg/ml PGA1. D. NBP2-PN54-C20 cells differentiated for 3 days with 200 µg/ml RO20-1724 and 2 mM dibutyryl cAMP. E. NBP2-PN54-C20 cells differentiated for 3 days with 200 µg/ml RO20-1724 and 2 µg/ml PGA1 in the presence of 5 µg/ml selenomethionine.
