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Antioxidant Supplementation Effects on Low-Density Lipoprotein Oxidation for Individuals with Type 2 Diabetes Mellitus

James W. Anderson, MD, Maya S. Gowri, PhD, Jan Turner, RD, Laura Nichols, RN, Veda A. Diwadkar, MSc, Ching K. Chow, PhD and Peter R. Oeltgen, PhD

Metabolic Research Group, VA Medical Center and Department of Internal Medicine, College of Medicine (J.W.A., M.S.G., J.T., L.N., V.A.D.), University of Kentucky, Lexington, Kentucky
Department of Nutrition and Food Science (C.K.C.), University of Kentucky, Lexington, Kentucky
Department of Pathology (P.R.O.), University of Kentucky, Lexington, Kentucky



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Fig. 1. Conjugated diene formation with Cu oxidation at 30°C of LDL from diabetic subjects. Samples from five diabetic subjects before (x) and after ({triangleup}) antioxidant supplementation for 12 weeks are illustrated.

 


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Fig. 2. Decrease in free amine groups in LDL with Cu oxidation at 37°C for control subjects ({blacksquare}) and diabetic subjects before ({circ}) and after (•) antioxidant supplementation for 12 weeks.

 


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Fig. 3. Uptake by mouse peritoneal macrophages of minimally-modified LDL from control subjects ({blacksquare}) and diabetic subjects before (•) and after ({circ}) antioxidant supplementation for 12 weeks. LDL, 10 to 50 µg/mL, were incubated for two hours with cells; DiI was extracted, measured by spectrofluorometry and reported as µg LDL protein/mg cell protein.

 





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