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Fig. 1. Flow chart for the assessment of potential allergencity of crops derived through biotechnology [7]. The series of steps used to assess the allergenicity of proteins isolated from plants that contain known allergens flows down the left side of the chart. The abbreviation DBPCFC in the box in the lower left corner stands for double-blind placebo-controlled food challenge. The protocol must be approved by an Human Subjects Institutional Review Board (IRB). The pathway down the right side of the figure shows the steps that are used to evaluate potential allergenicity of a protein isolated from a plant not known to cause food allergy.
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