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Fig. 2. Effect of vitamin C on growth of human tumorigenic parotid acinar cells in culture (2HPG1). Cells (100,000/60 mm dish) were plated in tissue culture dishes, and freshly prepared sodium ascorbate solution at various concentrations was added 24 hours later. Freshly prepared sodium ascorbate solution and medium were changed after two days of treatment. Growth was determined after three days of treatment. Each value (mean ± SEM) represents an average of nine samples [16].